Characterization of a weak allele of the GL1 gene of Arabidopsis thaliana

• Published in: Plant molecular biology Vol. 24; no. 1; pp. 203 - 207
• Main Authors: Esch, J.J. (Nebraska-Lincoln Univ., Lincoln, NE (USA). School of Biological Sciences), Oppenheimer, D.G, Marks, M.D
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-01-1994
• Subjects: Alleles; Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - ultrastructure; Arabidopsis Proteins; ARABIDOPSIS THALIANA; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA; DNA-Binding Proteins; Exons; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Plant; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Plant Proteins - genetics; PLANT TISSUES; SECUENCIA NUCLEICA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; SEQUENCE NUCLEIQUE; TEJIDOS VEGETALES; TISSU VEGETAL; Nucleotide sequence; Sequence alignment; Homology; Trichome; Arabidopsis thaliana; Allele; Cruciferae; Dicotyledones; Angiospermae; Development; Spermatophyta; Transcription factor; Experimental plant; Comparative study; Aminoacid sequence
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00040586

A genomic clone containing the gl1-2 allele has been isolated and sequenced. The predicted amino acid sequence of the gl1-2 protein is identical to that of the GL1-Col allele up to position 201. At this point in the coding region of gl1-2 there is a deletion relative to the wild-type sequence that results in an in-frame stop codon at position 202. This deletion removes 27 amino acid residues, including a highly negatively charged region, from the predicted gl1-2 polypeptide. The loss of this negatively charged carboxy-terminal region from the gl1-2 product is most likely the cause of the partial loss of gene activity which results in a reduction in leaf trichome initiation.