Plasma levels of gh and prl and concentrations in the fluids of bovine ovarian cysts and follicles

• Published in: Theriogenology Vol. 49; no. 7; pp. 1377 - 1387
• Main Authors: Borromeo, V., Berrini, A., Bramani, S., Sironi, G., Finazzi, M, Secchi, C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-05-1998
• Subjects: Animals; bovine; Cattle - physiology; Cattle Diseases - metabolism; Cattle Diseases - pathology; Estradiol - analysis; Female; Growth Hormone - analysis; Growth Hormone - blood; Growth Hormone - secretion; Immunoenzyme Techniques - veterinary; Immunohistochemistry; Ovarian Cysts - chemistry; Ovarian Cysts - pathology; Ovarian Cysts - secretion; Ovarian Cysts - veterinary; Ovarian Follicle - chemistry; Ovarian Follicle - cytology; Ovarian Follicle - secretion; ovary; plasma; PRL; Progesterone - analysis; Prolactin - analysis; Prolactin - blood; Prolactin - secretion; Seasons; Statistics, Nonparametric; GH; bovine; ovary; PRL; plasma
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/S0093-691X(98)00084-3

Prolactin and GH have been detected within the ovary, and it has become increasingly evident that they have a role as intrafollicular regulatory factors. The aim of the present work was to gain an insight into the elements influencing intraovarian GH and PRL in bovine species and to see whether cystic degeneration was accompanied by abnormal bovine GH (bGH) and PRL (bPRL) plasma patterns. We followed the relationships between plasma and ovarian fluid bGH and bPRL concentrations over an entire year in Friesian cows whose ovaries showed distinct types of structures. To assess the presence of bGH and bPRL within ovarian cells, we assayed selected ovarian structures by immunohistochemistry. The results demonstrated that: 1) plasma and ovarian fluid hormonal concentrations were independent, and their ratio was independent of the ovarian structure classes, subclasses and period of the year; 2) in the majority of the cows the concentration of bGH in ovarian fluid was no more than 80% of the level in plasma, whereas in about half the animals bPRL concentrations were higher in the ovary than in peripheral plasma; 3) mean bPRL concentrations in ovarian fluids were significantly higher in summer than in winter; 4) immunoreactive bGH and bPRL were present within granulosa and luteal cells. Thus, it is suggested that in the cow bGH and bPRL levels in the ovary might be regulated in some way independently of the pituitary.