The influence of strontium release rate from bioactive phosphate glasses on osteogenic differentiation of human mesenchymal stem cells

• Published in: Journal of the European Ceramic Society Vol. 38; no. 3; pp. 887 - 897
• Main Authors: Stefanic, M., Peroglio, M., Stanciuc, A.-M., Machado, G.C., Campbell, I., Kržmanc, M. Maček, Alini, M., Zhang, X.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2018
• Subjects: Bioactive glass; Biodegradable; Bone regeneration; Human mesenchymal stem cells; Phosphate glass; Strontium; Strontium; Bioactive glass; Human mesenchymal stem cells; Biodegradable; Phosphate glass; Bone regeneration
• ISSN: 0955-2219; 1873-619X
• DOI: 10.1016/j.jeurceramsoc.2017.08.005

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Strontium-doped bioactive phosphate glasses (P-glasses) of general composition 40(P2O5)–25(CaO)–5(Na2O)–(30-x)MgO–x(SrO) (x=0,1,5,10) were fabricated via melt-quenching technique and effects of strontium (Sr) content on P-glass properties were systematically studied, including glass structure and density, thermal properties, solubility and cytocompatibility with human mesenchymal stem cells (hMSCs). Sr-doping resulted in the weakening of glass network and a decrease in glass transition and melting temperatures. P-glasses maintained physiological pH on immersion in simulated body fluid and exhibited sustained ion release for up to ten weeks. All P-glasses tested were cytocompatible with hMSCs. Sr2+ release upregulated the genes associated with hMSC osteogenic differentiation; expression levels of early markers (RUNX2, COL 1) were proportional to the amount of Sr2+ in the P-glasses, while expression of late markers (AlP, OC) was the highest for the P-glass containing 5mol% Sr. It was concluded that Sr-doped P-glasses promoted osteogenic differentiation of hMSCs and have considerable potential for bone tissue regeneration.