Interferon-γ Inhibits Nonopsonized Phagocytosis of Macrophages via an mTORC1-c/EBPβ Pathway

• Published in: Journal of innate immunity Vol. 7; no. 2; pp. 165 - 176
• Main Authors: Wang, Zengfu, Zhou, Shuping, Sun, Chenming, Lei, Tong, Peng, Jianxia, Li, Weiguo, Ding, Pengbo, Lu, Jun, Zhao, Yong
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01-01-2015
• Subjects: Animals; Antibodies, Blocking - pharmacology; CCAAT-Enhancer-Binding Protein-beta - metabolism; Chick Embryo; Chickens; Dogs; Erythrocytes - immunology; Influenza A virus - immunology; Interferon-gamma - metabolism; Macrophages - immunology; Madin Darby Canine Kidney Cells; Mechanistic Target of Rapamycin Complex 1; Mice; Mice, Inbred C57BL; Multiprotein Complexes - metabolism; Orthomyxoviridae Infections - metabolism; Phagocytosis; Receptors, Immunologic - metabolism; Research Article; Signal Transduction; TOR Serine-Threonine Kinases - metabolism; Host defense; Cytokines; Macrophages; Phagocytosis
• ISSN: 1662-811X; 1662-8128
• DOI: 10.1159/000366421

Bacterial infection often follows virus infection due to pulmonary interferon-γ (IFN-γ) production during virus infection, which down-regulates macrophage phagocytosis. The molecular mechanisms for this process are still poorly understood. In the present study, IFN-γ treatment significantly inhibited the ability of mouse macrophages to phagocytize nonopsonized chicken red blood cells (cRBCs), bacteria and beads in vitro, while it enhanced IgG- and complement-opsonized phagocytosis. IFN-γ treatment decreased the expression of MARCO (macrophage receptor with collagenous structure) in macrophages. Macrophages showed lower binding to and phagocytic ability of cRBCs when MARCO was blocked with antibody. In addition, IFN-γ induced high activity of mTOR (mammalian target of rapamycin) and decreased the expression of c/EBPβ (CCAAT enhancer-binding protein β) in macrophages. Rapamycin, a specific mTOR inhibitor, significantly reversed the inhibitory effect of IFN-γ on nonopsonized phagocytosis of macrophages and restored c/EBPβ and MARCO expression. Biochemical assays showed that c/EBPβ directly bound to the MARCO gene promoter. Rapamycin significantly hampered the viral-bacterial synergy and protected influenza-infected mice from subsequent bacterial infection. Thus, IFN-γ inhibited the nonopsonized phagocytosis of macrophages through the mTOR-c/EBPβ-MARCO pathway. The present study offered evidence indicating that mTOR may be one of the key target molecules for the prevention of secondary bacterial infection caused by primary virus infection.