Explore the underlying oral efficacy of α-, β-, γ-Cyclodextrin against the ulcerative colitis using in vitro and in vivo studies assisted by network pharmacology

• Published in: Journal of biomolecular structure & dynamics Vol. 42; no. 10; pp. 4985 - 5000
• Main Authors: Wang, Weiqin, Li, Xuefeng, Wu, Hangyi, Shi, Fanli, Zhang, Zhenhai, Lv, Huixia
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-07-2024
• Subjects: acute ulcerative colitis; Administration, Oral; Animals; anti-inflammatory; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; beta-Cyclodextrins - chemistry; beta-Cyclodextrins - pharmacology; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Cyclodextrin; Cyclodextrins - chemistry; Cyclodextrins - pharmacology; Dextran Sulfate; Disease Models, Animal; Gastrointestinal Microbiome - drug effects; intestinal flora; Male; Mice; Network Pharmacology; RAW 264.7 Cells; anti-inflammatory; Cyclodextrin; network pharmacology; acute ulcerative colitis; intestinal flora
• ISSN: 0739-1102; 1538-0254
• DOI: 10.1080/07391102.2023.2239901

The incidence of ulcerative colitis (UC) is rising worldwide. As a refractory and recurrent disease, UC could seriously affect the patients' quality of life. However, current clinical medical treatments for UC are accompanied by various side effects, especially for long-term applications. Here, the underlying efficacy of cyclodextrins (CDs) was studied. As common excipients, CDs endow proven safety for long-term applications. Results of predictive methods derived from network pharmacology prompted the potential anti-inflammatory effects of CDs by oral administration. RAW264.7 cell experiments verified that CDs could inhibit the excessive secretion of TNF-α (β-CD > α-CD ≈ γ-CD), IL-6, and NO (α-CD > β-CD ≈ γ-CD) as predicted. In mice with DSS-induced acute UC, oral administration of CDs could effectively mitigate the pathological damage of colon tissue and reduce the level of inflammatory mediators. Moreover, 16S rRNA sequencing displayed that gut microbes disturbed by DSS were significantly regulated by CDs. Conclusively, the study showed the therapeutic application prospects of CDs in UC treatment and indicated the feasibility and advantages of developing 'new' therapeutic activities of 'old' ingredients.
Communicated by Ramaswamy H. Sarma