Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-ca...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) Vol. 52; no. 12; p. 2477
Main Authors: An, Heeseon, Statsyuk, Alexander V
Format: Journal Article
Language:English
Published: England 01-01-2016
Subjects:
Catalysis
Enzymes - chemistry
Molecular Probes - chemical synthesis
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Summary:We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.
ISSN:1364-548X
DOI:10.1039/c5cc08592f