Intercept-PCR, an improvement for elevating performance to find a new member of a certain gene family

• Published in: Molecular biotechnology Vol. 16; no. 1; pp. 1 - 4
• Main Authors: MATSUDA, Satoru, ICHIGOTANI, Yasukatu, OKUDA, Takahito, MIYAZAKI, Kou, YAMAMOTO, Tatsuyoshi, NIMURA, Yuji, IRIMURA, Tatsuro, NAKATSUGAWA, Shigekazu, HAMAGUCHI, Michinari
• Format: Journal Article
• Language: English
• Published: Totowa, NJ Humana Press 01-09-2000; Springer Nature B.V
• Subjects: Base Sequence; Biological and medical sciences; Cell Line; Cloning; Diverse techniques; DNA Primers; DNA, Complementary; Fundamental and applied biological sciences. Psychology; Genes; Humans; Molecular and cellular biology; Multigene Family; Polymerase chain reaction; Polymerase Chain Reaction - methods; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Sensitivity and Specificity; Polymerase chain reaction; Reverse transcription; Multigene family; Method; Gene; Molecular cloning
• ISSN: 1073-6085; 1559-0305; 1073-6085
• DOI: 10.1385/MB:16:1:01

We have established a method by which the performance of reverse transcriptase coupled polymerase chain reaction (RT-PCR) for seeking a new gene is improved. The actual procedure is quite easy: it is only to add several specific oligonucleotides into the reaction mixture of the usual RT-PCR. To verify the effectiveness of this method is also easy: it is only to detect the PCR products in the preliminary experiment. The finding in the present study provides valuable information for gene cloning tactics.