Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

• Published in: Turkish neurosurgery Vol. 22; no. 2; pp. 207 - 217
• Main Authors: Karaoz, Erdal, Kabatas, Serdar, Duruksu, Gokhan, Okcu, Alparslan, Subasi, Cansu, Ay, Birol, Musluman, Murat, Civelek, Erdinc
• Format: Journal Article
• Language: English
• Published: Turkey 2012
• Subjects: Animals; Biomarkers - metabolism; Bone Marrow Transplantation - methods; Cells, Cultured; Disease Models, Animal; Female; Green Fluorescent Proteins - genetics; Laminectomy - methods; Lumbar Vertebrae; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Motor Activity - physiology; Rats; Rats, Wistar; Recovery of Function - physiology; Spinal Cord Injuries - pathology; Spinal Cord Injuries - physiopathology; Spinal Cord Injuries - therapy; Tissue and Organ Harvesting - methods; Wound Healing - physiology
• ISSN: 1019-5149
• DOI: 10.5137/1019-5149.JTN.5412-11.1

This study aimed to analyze the effect of rat bone marrow-mesenchymal stem cells (rBM-MSCs) delivery on lesion site after spinal cord injury, and to observe the functional recovery after transplantation.
MSCs were isolated from rat femurs and tibias. The experimental rat population was divided into four groups: only laminectomy (1); laminectomy+trauma (2); laminectomy+trauma+PBS (3); laminectomy+trauma+MSCs (4). Their motility were scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords were examined for GFP labeled rBM-MSCs by immunostainings.
In the early posttraumatic period, the ultrastructures of spinal cord tissue were preserved in Group 4. The majority of cells forming the ependymal region around the central canal were found to be MSCs. The gray-and-white-matter around the ependymal region were composed of Nestin+/GFAP+ cells, with astrocytic-like appearance. The scores showed significant motor recovery in Group 4, especially in hind limb functions. However, no obvious change was observed in other groups.
The increase Nestin+/GFAP+ cells in the gray-and-white-matter around the ependymal region could indicate the potential to self-renew and plasticity. Thus, transplantation of rBM-MSCs might be an effective strategy to improve functional recovery following spinal cord trauma. In conclusion, molecular factors in cell fate decisions could be manipulated to enhance reparative potential of cell-based therapy.