Does sucrose influence the properties of DMPC vesicles?

• Published in: Chemistry and physics of lipids Vol. 123; no. 1; pp. 31 - 44
• Main Authors: Kiselev, M.A., Wartewig, S., Janich, M., Lesieur, P., Kiselev, A.M., Ollivon, M., Neubert, R.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01-03-2003
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Calorimetry, Differential Scanning; Lipid Bilayers - chemistry; Liposomes - chemistry; Macromolecular Substances; Membrane; Membrane Fluidity; Membrane Microdomains - chemistry; Molecular Conformation; Phospholipid; Raman spectroscopy; Small-angle scattering; Spectrum Analysis, Raman; Sucrose; Sucrose - chemistry; Temperature; Vesicle; Water - chemistry; X-Ray Diffraction; Vesicle; Phospholipid; Membrane; Sucrose; Small-angle scattering; Raman spectroscopy
• ISSN: 0009-3084; 1873-2941
• DOI: 10.1016/S0009-3084(02)00140-8

Small-angle neutron and X-ray scattering, dynamic light scattering, X-ray diffraction coupled with differential scanning calorimetry, and Raman spectroscopy were applied to investigate unilamellar (ULVs) and multilamellar (MLVs) dimyristoylphosphatidylcholine (DMPC) vesicles in aqueous sucrose solutions with sucrose concentrations from 0 to 60% w/w. In case of ULVs, the addition of sucrose decreases the polydispersity of vesicle population. A minimum value of polydispersity was found at 20% sucrose. For sucrose concentration from 0 to 35% oligolamellar vesicles in the ULV population have a minimum presence. Vesicles with 5–10% sucrose exhibit the best stability in time. For the case of MLVs, sucrose influences the temperature of the phase transitions, but the internal membrane structure remains unchanged.