Effects of metamizol and magnesium sulfate on enzyme activity of glucose 6-phosphate dehydrogenase from human erythrocyte in vitro and rat erythrocyte in vivo

• Published in: Clinical biochemistry Vol. 34; no. 4; pp. 297 - 302
• Main Authors: Çiftçi, Mehmet, Özmen, İsmail, Büyükokuroğlu, M.Emin, Pençe, Sadrettin, Küfrevioğlu, Ö.İrfan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-06-2001
• Subjects: Ammonium Sulfate - pharmacology; Animals; Anti-Arrhythmia Agents - pharmacology; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Chromatography, Affinity; Dipyrone - pharmacology; Electrophoresis, Polyacrylamide Gel; Erythrocytes - drug effects; Erythrocytes - enzymology; Glucose 6-phosphate dehydrogenase; Glucosephosphate Dehydrogenase - blood; Glucosephosphate Dehydrogenase - metabolism; Human; Humans; Inhibitory Concentration 50; Kinetics; Magnesium sulfate; Magnesium Sulfate - pharmacology; Male; Metamizol; Rat; Rats; Rats, Sprague-Dawley; Metamizol; Human; Magnesium sulfate; Rat; Glucose 6-phosphate dehydrogenase
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/S0009-9120(01)00207-7

Objective: Effects of metamizol and magnesium sulfate on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in
in vitro and
in vivo conditions.
Methods: For
in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for
in vivo studies. Enzyme activity was determined according to the Beutler method by using a spectrophotometer at 340 nm.
Results: The results of
in vitro study showed that their mean K
i values were 6.35 × 10
−3 M for metamizol and 1.32 × 10
−2 M for magnesium sulfate and their inhibition types were uncompetitive. I
50 value was 17 mM for metamizol and 50 mM for magnesium sulfate in
in vitro study. In the case of
in vivo studies, 200 mg/kg metamizol inhibited the enzyme activity by 40% during the first 1.5 h (
p < 0.05), and 225 mg/kg magnesium sulfate significantly inhibited the enzyme activity throughout 24 h (
p < 0.01).
Conclusion: The results of this study suggested that metamizol and magnesium sulfate have significant inhibition effect on the activity of glucose 6-phosphate dehydrogenase enzyme in both
in vivo and
in vitro.