Molecular cloning of the PEL1 gene of Saccharomyces cerevisiae that is essential for the viability of petite mutants

• Published in: Current genetics Vol. 24; no. 4; pp. 307 - 312
• Main Authors: Janitor, M. (Comenius Univ., Bratislava (Slovak Republic). Dept. of Microbiology and Virology), Subik, J
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-10-1993; Berlin
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; CARTE GENETIQUE; CDPdiacylglycerol-Serine O-Phosphatidyltransferase; Classical genetics, quantitative genetics, hybrids; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; Codon; CONTROLE DE CROISSANCE; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; GENE; GENES; Genes, Fungal; Genetic Complementation Test; Genetic Linkage; Genetic mapping; Genetics of eukaryotes. Biological and molecular evolution; Growth control; Invertebrata; MAPAS GENETICOS; MITOCHONDRIE; MITOCONDRIA; Molecular cloning; Molecular Sequence Data; MUTANT; MUTANTES; Mutation; NOYAU CELLULAIRE; NUCLEO; Nucleo-mitochondrial interaction; Phenotype; REGULACION DEL CRECIMIENTO; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins; Temperature; Viability of petites; Fungi; Genetic mapping; Cell proliferation; Yeast; Nucleotide sequence; Chromosome DNA; Ascomycetes; Nucleocytoplasmic interaction; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00336781

The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome 3, centromere proximal of LEU2. The PEL1 gene has been isolated and cloned by the complementation of a pel1 mutation. The molecular analysis of the chromosomal insert carrying PEL1 revealed that this gene corresponds to the YCL4W open reading frame on the complete DNA sequence of chromosome 3. The putative Pel1 protein is characterized by a low molecular weight of approximately 17 kDa, a low codon adaptation index, and a high leucine content.