Magainin II modified polydiacetylene micelles for cancer therapy

Polydiacetylene (PDA) micelles have been widely used to deliver anticancer drugs in the treatment of a variety of tumours and for imaging living cells. In this study, we developed an effective strategy to directly conjugate magainin II (MGN-II) to the surface of PDA micelles using a fluorescent dye....

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Bibliographic Details
Published in:	Nanoscale Vol. 6; no. 24; p. 14772
Main Authors:	Yang, Danling, Zou, Rongfeng, Zhu, Yu, Liu, Ben, Yao, Defan, Jiang, Juanjuan, Wu, Junchen, Tian, He
Format:	Journal Article
Language:	English
Published:	England 21-12-2014
Subjects:	Animals Antineoplastic Agents - administration & dosage Antineoplastic Agents - chemistry Apoptosis - drug effects Cell Line, Tumor Diffusion Drug Design Fluorescent Dyes - chemistry Magainins - administration & dosage Magainins - chemistry Male Mice Mice, Inbred BALB C Mice, Nude Micelles Microscopy, Fluorescence - methods Nanocapsules - administration & dosage Nanocapsules - chemistry Nanocapsules - ultrastructure Neoplasms, Experimental - drug therapy Neoplasms, Experimental - pathology Particle Size Polyacetylenes - chemistry Polymers - chemistry Treatment Outcome Xenopus Proteins - administration & dosage Xenopus Proteins - chemistry
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Summary:	Polydiacetylene (PDA) micelles have been widely used to deliver anticancer drugs in the treatment of a variety of tumours and for imaging living cells. In this study, we developed an effective strategy to directly conjugate magainin II (MGN-II) to the surface of PDA micelles using a fluorescent dye. These stable and well-defined PDA micelles had high cytotoxicity in cancer cell lines, and were able to reduce the tumour size in mice. The modified PDA micelles improved the anticancer effects of MGN-II in the A549 cell line only at a concentration of 16.0 μg mL(-1) (IC50). In addition, following irradiation with UV light at 254 nm, the PDA micelles gave rise to an energy transfer from the fluorescent dye to the backbone of PDA micelles to enhance the imaging of living cells. Our results demonstrate that modified PDA micelles can not only be used in the treatment of tumors in vitro and in vivo in a simple and directed way, but also offer a new platform for designing functional liposomes to act as anticancer agents.
ISSN:	2040-3372
DOI:	10.1039/c4nr04405c