Ex vivo investigation of the use of hydrothermal energy to induce chondrocyte necrosis in articular cartilage of the metacarpophalangeal and metatarsophalangeal joints of horses

• Published in: American journal of veterinary research Vol. 66; no. 1; pp. 36 - 42
• Main Authors: Jenner, F, Edwards, R.B. III, Voss, J.R, Southwood, L, Markel, M.D, Richardson, D.W
• Format: Journal Article
• Language: English
• Published: United States 2005
• Subjects: Animals; arthrodesis; Arthrodesis - methods; Arthrodesis - veterinary; articular cartilage; cartilage; Cartilage, Articular - cytology; Cell Survival; Chondrocytes; confocal scanning laser microscopy; differential staining; Forelimb; Hindlimb; histology; horse diseases; Horses; Hot Temperature; hot water treatment; joint diseases; joints (animal); Joints - anatomy & histology; lavage; medical treatment; metacarpus; metatarsus; Necrosis; Therapeutic Irrigation - veterinary; viability; washing
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.2005.66.36

To evaluate the use of hydrothermal ablation of articular cartilage for arthrodesis in horses through investigation of the effects of joint lavage with physiologic saline (0.9% NaCI) solution (80 degrees C) for various treatment times on chondrocyte viability in the articular cartilage of the metacarpophalangeal and metatarsophalangeal joints of cadaveric horse limbs. Sample Population-7 pairs of metacarpophalangeal and 8 pairs of metatarsophalangeal joints from 8 Thoroughbreds.
The horses were euthanatized for reasons unrelated to musculoskeletal disease. On a random basis, 1 joint of each pair underwent intra-articular lavage for 5, 10, or 15 minutes with heated saline solution (80 degrees C); the other joint underwent sham treatment of similar duration with saline solution at 22 degrees C (control). Cartilage samples from the distal articular surface of metacarpus III (or metatarsus III), the proximal surface of the proximal phalanx, and the lateral and medial proximal sesamoid bones were assessed for chondrocyte viability via confocal microscopy and viability staining following enzymatic digestion.
Compared with the control joints, findings of both viability assays indicated that the percentage of sites containing viable chondrocytes in heat-treated joints was decreased. Treatment hazard ratios of 0.048 (confocal microscopy) and 0.2 (digestion assay) were estimated. Histologically, periarticular soft tissues had minimal detrimental effects after heat treatment.
Ex vivo intra-articular lavage with saline solution at 80 degrees C resulted in the death of almost all articular chondrocytes in the joint. This technique may be a satisfactory method for extensive cartilage ablation when performing arthrodesis by minimally invasive techniques.