Heterologous Kluyveromyces lactisβ-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants

• Published in: Biochimica et biophysica acta Vol. 1335; no. 3; pp. 235 - 241
• Main Authors: Becerra, M, Cerdán, E, González Siso, M.I
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06-06-1997
• Subjects: beta-Galactosidase - biosynthesis; beta-Galactosidase - economics; beta-Galactosidase - genetics; Cell Wall - metabolism; Hot Temperature; Kluyveromyces - enzymology; Mutation; Plasmids; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Yeast autolytic mutant; β-Galactosidase; Yeast autolytic mutant; β-Galactosidase
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(97)00048-2

The
β-galactosidase from
Kluyveromyces lactis is a high molecular weight protein with commercial interest. A major drawback of its industrial production is the high cost associated with extraction and downstream processing due to its intracellular nature. In this work, the effectiveness of the utilization of
Saccharomyces cerevisiae LD1 and LHDP1 strains, osmotic-remedial mutants which lyse at 37°C, for the heterologous production and release into the extracellular medium of this protein has been proved. The highest absolute values of released
β-galactosidase have been obtained with the protease-deficient strain LHDP1 by osmotic shock.