Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR)

• Published in: European cytokine network Vol. 28; no. 2; pp. 63 - 69
• Main Authors: Vilia, Maria Giovanna, Tocchetti, Marta, Fonte, Eleonora, Sana, Ilenia, Muzio, Marta
• Format: Journal Article
• Language: English
• Published: France Springer Nature B.V 01-06-2017
• Subjects: Alternative Splicing; Cell Line; Cell lines; Cloning, Molecular; Exons; Fluorescent Antibody Technique; Gene Expression Regulation; Humans; Inflammation; Interleukin 1; Interleukin 1 receptors; Isoforms; Localization; Membrane proteins; mRNA; Protein Isoforms; Proteins; Receptors, Interleukin-1 - genetics; Receptors, Interleukin-1 - metabolism; RNA, Messenger - genetics; Signal Transduction; SIGIRR; IL-1R8; interleukin-1 receptor; splicing; TIR8; Toll-like receptor
• ISSN: 1148-5493; 1952-4005
• DOI: 10.1684/ecn.2017.0395

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.