Rhizobium-legume symbiosis: observation of root infection by bright-field microscopy after staining with methylene blue

Staining of infected legume roots with 0.01% methylene blue facilitated the observation of the initial steps of the Rhizobium — legume symbiosis. It allowed particularly the visualization by bright-field microscopy of infection threads in the root hairs and the root cortex of the host plant.

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Bibliographic Details
Published in:	Planta Vol. 161; no. 6; pp. 487 - 489
Main Authors:	Vasse, J.M, Truchet, G.L
Format:	Journal Article
Language:	English
Published:	Berlin Springer-Verlag 01-01-1984 Springer
Subjects:	Agronomy. Soil science and plant productions Bacteria Biological and medical sciences Economic plant physiology Fundamental and applied biological sciences. Psychology Infections Laboratory staining techniques Legumes Microscopy Parasitism and symbiosis Plant physiology and development Plant roots Plants Rhizobium Root hairs soil biology soil science Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Leguminosae Dicotyledones Angiospermae Medicago sativa Bacteria Spermatophyta Plant nodule Rhizobium Rhizobiaceae Trifolium repens
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Description
Summary:	Staining of infected legume roots with 0.01% methylene blue facilitated the observation of the initial steps of the Rhizobium — legume symbiosis. It allowed particularly the visualization by bright-field microscopy of infection threads in the root hairs and the root cortex of the host plant.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-0935 1432-2048
DOI:	10.1007/BF00407080