Proteomic LC–MS systems using nanoscale liquid chromatography with tandem mass spectrometry

Current nano-scale liquid chromatography–tandem mass spectrometry (nanoLC–MS/MS) approaches in proteome research are reviewed from an analytical perspective. For comprehensive analysis of cellular proteins, analytical methods with higher resolution, sensitivity, and wider dynamic range are required....

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 1067; no. 1; pp. 73 - 83
Main Author:	Ishihama, Yasushi
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 04-03-2005
Subjects:	Chromatography, Liquid - methods Mass Spectrometry - methods NanoLC–MS/MS Nanotechnology Proteome Proteomics Proteome NanoLC–MS/MS Proteomics
Online Access:	Get full text
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Summary:	Current nano-scale liquid chromatography–tandem mass spectrometry (nanoLC–MS/MS) approaches in proteome research are reviewed from an analytical perspective. For comprehensive analysis of cellular proteins, analytical methods with higher resolution, sensitivity, and wider dynamic range are required. Miniaturized LC coupled with tandem mass spectrometry is currently one of the most versatile techniques. In this review, the current status of nanoLC–MS/MS systems as well as data management systems is addressed. In addition, the future prospects for complete proteomics are discussed.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2004.10.107