The Characteristics and Potential of Lactic Acid Bacteria as Probiotics in Silage Made from Hymenachne acutigluma and Neptunia oleracea lour

• Published in: E3S web of conferences Vol. 68; p. 1017
• Main Authors: Sandi, Sofia, Yosi, Fitra, Sari, Meisji Liana, Gofar, Nuni
• Format: Journal Article
• Language: English
• Published: EDP Sciences 01-01-2018
• ISSN: 2267-1242; 2267-1242
• DOI: 10.1051/e3sconf/20186801017

This study aims to determine the characteristics of lactic acid bacteria (LAB) obtained from the silage made from
Hymenachne acutigluma
and
Neptunia oleracea Lour
to be used as probiotics. This study used a completely randomized design (CRD), which consists of 3 treatments and 5 replications. The treatment consisted of P1 (silage made from
Hymenachne acutigluma
), P2 (silage made from 50% of
Hymenachne acutigluma
and 50% of
Neptunia oleracea lour
), and P3 (silage made from
Neptunia oleracea lour
). The variables measured were LAB characteristics including shape, gram staining, catalase, endospore, motility, growth at different temperatures, TSIA, MR, VP, and identification using API Kit. The data were analyzed descriptively. The results showed that there were 9 selected isolates. All isolates are rod-shaped, gram-positive, catalase, endospore, and negative motility, growth at 15 °C negative and at 45 °C positive. TSIA slant and butt test show yellow and negative colors on gas and H2S production, while MR-VP is red and yellow. The conclusions of this study were all isolates belonging to the
Lactobacillus plantarum
strain with a similarity level of 87.3-99.9 %.