In vitro antifungal susceptibility testing of fungi in patients with onychomycosis

• Published in: Dermatologic therapy Vol. 33; no. 3; pp. e13429 - n/a
• Main Authors: Abu El‐Hamd, Mohammed, Abd Elhameed, Manar Ibrahim, Shalaby, Mona Fattouh Mohamed, Saleh, Ramadan
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-05-2020
• Subjects: Antifungal Agents - pharmacology; Antifungal Agents - therapeutic use; antifungal susceptibility; antifungal target; Humans; Itraconazole - pharmacology; Naphthalenes - pharmacology; onychomycosis; Onychomycosis - diagnosis; Onychomycosis - drug therapy; Terbinafine; onychomycosis; antifungal susceptibility; antifungal target
• ISSN: 1396-0296; 1529-8019
• DOI: 10.1111/dth.13429

Onychomycosis is the most common nail disorder. To examine in vitro antifungal susceptibility of fungi among onychomycosis patients. The study included 68 patients with onychomycosis. Nail specimens were cultured on Sabouraud dextrose agar and Dermasel agar base‐media. Isolated fungi were subjected to antifungal susceptibility tests against terbinafine, itraconazole, fluconazole, and griseofulvin. Candida species (Candida spp.) were detected in 32.4% of the cases of candidal onychomycosis (n = 37), 23.5% of the cases of distal and lateral subungual onychomycosis (n = 17), and 21.4% of the cases of total dystrophic onychomycosis (n = 14). Candida spp. were sensitive to fluconazole in 73.5%, itraconazole in 58.8%, and terbinafine in 5.9% of the cases. Aspergillus spp. were sensitive to itraconazole in all cases, and terbinafine in 87.5% of cases. Penicillium spp. were sensitive to itraconazole and terbinafine in 88.9% and 77.8% of cases, respectively. Trichophyton spp. were sensitive to terbinafine and resistant to itraconazole. Microsporum spp. were sensitive to itraconazole and resistance to terbinafine. All isolated fungi were resistant to griseofulvin. An increasing proportion of Candida spp. was observed among patients with different clinical varieties of onychomycosis. Candida spp. were highly sensitive to fluconazole and a lesser extent to itraconazole.