Class I major histocompatibility complex-restricted T lymphocyte recognition of the influenza hemagglutinin: overlap between class I cytotoxic T lymphocytes and antibody sites

• Published in: The Journal of experimental medicine Vol. 170; no. 4; pp. 1357 - 1368
• Main Authors: SWEETSER, M. T, BRACIALE, V. L, BRACIALE, T. J
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01-10-1989; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Animals; Antigenic determinants, haptens, artificial antigens; Antigens; Biological and medical sciences; Epitopes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; H-2 Antigens - immunology; Hemagglutinins, Viral - immunology; HN Protein - immunology; influenza A virus; Influenza A virus - classification; Influenza A virus - immunology; Major Histocompatibility Complex; Mice; Mice, Inbred Strains; Molecular immunology; Molecular Sequence Data; Oligopeptides - immunology; Structure-Activity Relationship; T-Lymphocytes, Cytotoxic - immunology; Antigenic determinant; Nucleotide sequence; Hemagglutinin; Major histocompatibility system; Rodentia; Orthomyxoviridae; Cytotoxicity; Influenzavirus; Virus; Antigen; Vertebrata; Mammalia; Influenzavirus A; Mouse; T-Lymphocyte; Class I histocompatibility antigen; Recognition; Aminoacid sequence
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.170.4.1357

The influenza hemagglutinin is a critical regulator of disease expression during influenza virus infection and serves as a major target for the host immune response to this pathogen. In this report, we have analyzed an immunodominant site on the hemagglutinin (residues 202-221) recognized by murine class I MHC-restricted T lymphocytes. This analysis has revealed evidence for the duplication of a T cell recognition site within the region 202-221. We have also identified critical amino acids necessary for class I-restricted T cell recognition within these two epitopes. In addition, we provide evidence that a site on the influenza hemagglutinin recognized by neutralizing antibody directly overlaps with an epitope recognized by class I MHC-restricted CTL.