Simple synthesis route for fabrication of protective photo‐crosslinked poly(zwitterionic) membranes for application in non‐enzymatic glucose sensing

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 110; no. 3; pp. 679 - 690
• Main Authors: Olejnik, Adrian, Karczewski, Jakub, Dołęga, Anna, Siuzdak, Katarzyna, Cenian, Adam, Grochowska, Katarzyna
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-03-2022; Wiley Subscription Services, Inc
• Subjects: Acetaminophen; Acetylsalicylic acid; Ascorbic acid; Biofouling; Biomedical materials; Catalytic activity; Chemoreception; Coated electrodes; Crosslinking; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Fabrication; Fourier transforms; Glucose; Glucose - chemistry; glucose sensing; Glycine; Gold; Gold - chemistry; gold nanoparticles; Infrared spectroscopy; Materials research; Materials science; Membranes; Metal Nanoparticles - chemistry; Nanoparticles; Oxidation; Oxidation resistance; Oxidation-Reduction; PBS; poly(zwitterions); Polymers; Substrates; Synergistic effect; Urea; Zwitterions; PBS; membranes; poly(zwitterions); gold nanoparticles; glucose sensing
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.34946

This work focuses on the fabrication of non‐enzymatic glucose sensing materials based on laser‐formed Au nanoparticles embedded in Ti‐textured substrates. Those materials possess good catalytic activity toward glucose oxidation in 0.1 × phosphate buffered saline as well as resistance to some interferants, such as ascorbic acid, urea, and glycine. The electrodes are further coated with three different polymers, that is, Nafion, photo‐crosslinked poly(zwitterions) based on sulfobetaine methacrylate, and a hybrid membrane consisting of both polymers. Both the optimal integrity of the material and its catalytic activity toward glucose oxidation were maintained by the hybrid membranes with a large excess of poly(zwitterions) (mass ratio 20:1). The chemical structures of the as‐formed membranes are confirmed by Fourier transform infrared spectroscopy. Due to the zwitterionic nature of the coating, the electrodes are resistant to biofouling and maintain electrochemical activity toward glucose for 4 days. Moreover, due to the synergistic effect of both Nafion and poly(zwitterions), the interference from the two compounds, namely, from acetylsalicylic acid and acetaminophen, was diminished. Besides the presence of polymer membranes, the electrode possesses a sensitivity of 36.8 μA cm−2 mM−1 in the linear range of 0.4–12 mM, while the limit of detection was estimated to be 23 μM. Finally, the electrodes are stable, and their response is not altered even by 1,000 bending cycles.