Biochemistry of fern spore germination: protease activity in ostrich fern spores

• Published in: Plant physiology (Bethesda) Vol. 80; no. 4; pp. 992 - 996
• Main Authors: Cohen, H.P, DeMaggio, A.E
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-04-1986
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Amino acids; Biochemistry; Biological and medical sciences; Enzymes; ENZYMIC ACTIVITY; ESPORAS; Ferns; Fundamental and applied biological sciences. Psychology; GERMINACION; GERMINATION; Germination and dormancy; Homogenization; Imbibition; MATTEUCCIA STRUTHIOPTERIS; Plant physiology and development; PROTEASAS; PROTEASE; PROTEASES; PTERIDOPHYTA; SPORE; Spore germination; SPORES; Storage proteins; Pteridophyta; Enzyme; Germination; Proteinase; Spores; Filicineae
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.80.4.992

Protease activities were detected in quiescent and germinating spores of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Peak endopeptidase, aminopeptidase, and carboxypeptidase activities were detected 12 to 24 hours after spores began imbibing under light. There was a correlation between activities of proteases, the onset of a decline in levels of soluble protein, and an increase in levels of free amino acids. The earliest visible event of spore germination, breakage of the spore coat and protrusion of a rhizoid cell, was observed after peak protease activity, 48 to 72 hours after the start of imbibition. Results of this study demonstrate similarities in the pattern of protease activities during germination of ostrich fern spores to those of some seeds.