Renal Mechanisms Involved in Stress-induced Antinatriuresis and Antidiuresis in Rats

• Published in: Archives of physiology and biochemistry Vol. 111; no. 3; pp. 259 - 264
• Main Authors: Bertuzzi, M.L., Bensi, N., Mayer, N., Niebylski, A., Armario, A., Gauna, H.F.
• Format: Journal Article
• Language: English
• Published: Basingstoke Informa UK Ltd 01-07-2003; Taylor & Francis
• Subjects: Adrenergic alpha-Antagonists - pharmacology; Adrenergic beta-Antagonists - pharmacology; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Biological and medical sciences; Diuresis - drug effects; Diuresis - physiology; Fundamental and applied biological sciences. Psychology; Glomerular Filtration Rate; Immobilization; Keywords: a-adrenoceptor antagonist, captopril, rats, sodium excretion, immobilization stress; Kidney - drug effects; Kidney - physiology; Male; Natriuresis - drug effects; Natriuresis - physiology; Rats; Rats, Wistar; Receptors, Adrenergic, alpha - physiology; Receptors, Adrenergic, beta - physiology; Receptors, Angiotensin - physiology; Sodium - urine; Sodium Chloride, Dietary - pharmacology; Stress, Physiological - urine; Vertebrates: urinary system; Diuresis; Glomerular filtration; Rat; Immobilization; immobilization stress; Peptidyl-dipeptidase A; α-adrenoceptor antagonist; Antagonist; rats; Angiotensin II; sodium excretion; Excretion; Enzyme; Captopril; Rodentia; Enzyme inhibitor; Natriuria; Stress; Peptidases; Renin angiotensin system; Vertebrata; Mammalia; Sodium; α-Adrenergic receptor; Hydrolases; Peptidyl-dipeptidases; ACE inhibitor
• ISSN: 1381-3455; 1744-4160
• DOI: 10.1076/apab.111.3.259.23453

The present study was conducted to investigate if changes in sodium and water excretion in stressed animals were due to modifications in the glomerular filtration rate (GFR) and to determine the participation of angiotensin II (Ang II) and α and β-adrenoceptors on sodium and water renal excretion in rats subjected to immobilization stress (IMO). Male Wistar rats (250-300 g) were randomly separated into five different groups and vehicle (0.9% NaCl) via intraperitoneal (i.p.) or propanolol (3 mg/kg i.p.) or captopril (6 mg/kg i.p.) or yohimbine (3 mg/kg i.p.) or prazosin (1 mg/kg i.p.) were injected respectively. During experimental measurements, the animals were kept in metabolic cages for 6 h and sodium, potassium and water renal excretion and saline (1.5% NaCl) and water intake were determinated at day 1 (drug effect) and day 7 (drug + IMO effects). GFR was measured by creatinine clearance in control and IMO rats. A stress-induced antinatriuresis and antidiuresis was reversed by a 1 and a 2 -adrenoceptor antagonists, while captopril inhibited only the antidiuresis and propranolol had no effect on either parameter. No differences were observed in creatinine clearance in the studied groups. Since yohimbine blocks a 2 -adrenoceptors and prazosin blocks a 1 -adrenoceptors and a 2B -adrenoceptors, the stress-induced renal sodium reabsorption mainly could be attributed to a 2B -adrenoceptors. The present results indicate that β-adrenoceptors do not participate in this response and, Ang II only reverses the antidiuresis and shows a slight participation in antinatriuresis. The increment in sodium and water reabsorption caused by IMO occurred without changes in the glomerular filtration rate.