Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD)

An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected color...

Full description

Saved in:

Bibliographic Details
Published in:	Analyst (London) Vol. 126; no. 8; p. 1207
Main Authors:	Shimomura, M, Nomura, Y, Lee, K H, Ikebukuro, K, Karube, I
Format:	Journal Article
Language:	English
Published:	England 01-08-2001
Subjects:	Antibodies, Monoclonal Environmental Pollutants - analysis Enzyme-Linked Immunosorbent Assay - methods Hormones - analysis Polychlorinated Dibenzodioxins - analogs & derivatives Polychlorinated Dibenzodioxins - analysis Polychlorinated Dibenzodioxins - immunology
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected colorimetrically at 450 nm. The detection limit of OCDD was 0.78 pg mL(-1). Optimizing the reaction conditions of the assay, cross reactivities of some dioxins against the antibody are discussed.
ISSN:	0003-2654
DOI:	10.1039/b104258k