N-octanoylated ghrelin peptide inhibits bovine oocyte meiotic resumption

• Published in: General and comparative endocrinology Vol. 263; pp. 7 - 11
• Main Authors: Xu, X.L., Bai, J.H., Feng, T., Xiao, L.L., Song, Y.Q., Xiao, Y.X., Liu, Y.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2018
• Subjects: Bovine; Germinal vesicle breakdown; Meiotic resumption; N-octanoylated ghrelin; Oocyte maturation; Bovine; Meiotic resumption; Germinal vesicle breakdown; N-octanoylated ghrelin; Oocyte maturation
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2018.04.016

•50 ng/mL ghrelin could inhibit bovine oocytes germinal vesicle breakdown.•Increased cAMP and cGMP levels in CCs and DOs contributed to this inhibition.•Inhibitory ghrelin benefits the developmental competence of matured oocytes.•Ghrelin could act as a pharmaceutical meiosis inhibitor for bovine oocytes.
Studies have shown that ghrelin plays an important role in the mammalian reproductive system, including the central, gonadal levels, and also during in vitro maturation of oocytes; however, the functions of ghrelin in bovine oocyte meiosis require further investigation.
We aimed to evaluate the effects of an n-octanoylated ghrelin peptide on oocyte meiotic resumption and the developmental competence of mature oocytes in vitro.
design: The expression of GHRL (encoding ghrelin) mRNA and its receptor (the growth hormone secretagogue receptor, GHSR) in the cumulus-oocyte complex (COCs), denuded oocytes (DOs), and cumulus cells (CCs) was assessed using quantitative real-time reverse transcription PCR (qRT-PCR), and the effects of the n-octanoylated ghrelin peptide on meiotic resumption were studied at four different doses (0, 10, 50, and 100 ng/mL) in a 6 h culture system.
qRT-PCR analysis showed that GHRL and GHSR mRNAs were expressed in all tested samples; however, GHRL was predominantly expressed in DOs, and GHSR was predominantly expressed in CCs. Germinal vesicle breakdown was inhibited significantly by 50 ng/mL ghrelin compared with that in the negative control (P < 0.05). Further studies showed that n-octanoylated ghrelin increased the levels of cAMP and cGMP in the CCs and DOs, which inhibited the meiotic resumption of bovine oocytes. And the inhibitory role in the developmental competence of mature oocytes were also included, ghrelin could significantly improve the cleavage rate (P < 0.05) and blastocyst rate (P < 0.05).
N-octanoylated ghrelin maintained bovine oocytes meiotic arrest and further improved their developmental competence; therefore, n-octanoylated ghrelin could be considered as a potential pharmaceutical inhibitor of meiosis for the in vitro maturation of bovine oocytes.