Electrophoretically deposited nano-structured polyaniline film for glucose sensing

• Published in: Thin solid films Vol. 519; no. 3; pp. 1145 - 1150
• Main Authors: Dhand, Chetna, Sumana, G., Datta, Monika, Malhotra, B.D.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 30-11-2010; Elsevier
• Subjects: Biosensor; Condensed matter: structure, mechanical and thermal properties; Conducting polymers; Cross-disciplinary physics: materials science; rheology; Deposition; Electrophoretic deposition; Enzymes; Exact sciences and technology; Glucose; Glucose oxidase; Indium tin oxide; Materials science; Nano-structured polyaniline; Nanocomposites; Nanomaterials; Nanoscale materials and structures: fabrication and characterization; Nanostructure; Other topics in nanoscale materials and structures; Physics; Polyanilines; Structure and morphology; thickness; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Thin film structure and morphology; Conducting polymers; Glucose oxidase; Biosensor; Nano-structured polyaniline; Electrophoretic deposition; Enzymes; Scanning electron microscopy; Immobilization; Glucose; Nanostructures; Indium oxide; Thin films; Tin oxide; Biosensors; Polyanilines
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2010.08.059

Electrophoretically deposited nano-structured polyaniline (NS-PANI) film has been utilized for fabrication of glucose biosensor by covalent immobilization of glucose oxidase (GOx) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. This GOx/NS-PANI/ITO bioelectrode has been characterized using scanning electron microscopy, FT-IR, UV–Visible spectroscopy and differential pulse voltammetry (DPV) techniques. The response studies carried out on GOx/NS-PANI/ITO bioelectrode using DPV and photometric studies reveal linearity up to 400
mgdL
−
1
with sensitivity as 1.05
×
10
−
4
mA
mg
−
1
dL and 3.887
×
10
−
5
Abs
mg
−
1
dL, respectively. The lower value of Michaelis–Menten constant obtained for immobilized GOx (2.1
mM) compared with that of free GOx (5.85
mM) suggests high affinity of enzyme to this matrix.