STC1 and NF-κB p65 (Rel A) is Constitutively Activated in Colorectal Cancer

• Published in: Clinical laboratory (Heidelberg) Vol. 62; no. 3; p. 463
• Main Authors: Rezapour, Saleheh, Bahrami, Tayyeb, Hashemzadeh, Shahryar, Estiar, Mehrdad Asghari, Nemati, Masoumeh, Ravanbakhsh, Reyhaneh, Feizi, Mohammad Ali Hosseinpour, Kafil, Hossein Samadi, Pouladi, Nasser, Ghojazadeh, Morteza, Sakhinia, Ebrahim
• Format: Journal Article
• Language: English
• Published: Germany 2016
• Subjects: Adult; Aged; Colorectal Neoplasms - etiology; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Female; Glycoproteins - genetics; Humans; Male; Middle Aged; RNA, Messenger - analysis; Transcription Factor RelA - genetics
• ISSN: 1433-6510
• DOI: 10.7754/Clin.Lab.2015.150827

Stanniocalcin-1 (STC1) and nuclear factor (NF)-κB subunit p65 transcription factor are involved in various types of human malignancies. The roles of STC1 and NFκB-p65 in colorectal cancer (CRC) are still not fully understood. We investigated expression levels of NF-κB p65 and STC1 and also correlations between STC1 and NF-κB p65 expression and clinicopathological features in CRC.
Tumor tissue samples were collected from 48 patients with CRC. RT-PCR and Real-time PCR analysis was performed to examine mRNA levels of STC1 and NF-κB p65.
The relative mRNA levels of STC1 and NF-κB p65 were significantly higher in tumor tissues than in adjacent mucosa (p = 0.025 and p = 0.044, respectively). The data also showed that STC1 and NF-κB p65 mRNA levels were not significantly associated with clinicopathological characteristics. In addition, there was no association between expression levels of STC1 and NF-κB p65 in tumor samples.
Our data indicate that STC1 and NF-κBp65 is activated constitutively in colorectal carcinoma tissues, suggesting that activation of these factors might play an important role in colorectal tumorigenesis. Future studies should examine STC1 and NF-κBp65 as a molecular target for the treatment of CRC.