Germination of Arabidopsis Seed in Space and in Simulated Microgravity: Alterations in Root Cell Growth and Proliferation

• Published in: Microgravity science and technology Vol. 21; no. 4; pp. 293 - 297
• Main Authors: Manzano, Ana I., Matía, Isabel, González-Camacho, Fernando, Carnero-Díaz, Eugénie, van Loon, Jack J. W. A., Dijkstra, Camelia, Larkin, Oliver, Anthony, Paul, Davey, Michael R., Marco, Roberto, Medina, F. Javier
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-11-2009; Springer Nature B.V
• Subjects: Aerospace Technology and Astronautics; Cell cycle; Cell growth; Classical and Continuum Physics; Engineering; Gene expression; Germination; Original Article; Plant growth; Proteins; Space Exploration and Astronautics; Space Sciences (including Extraterrestrial Physics; Space stations; Cell proliferation; Cell growth; Immunogold; GUS; Cell cycle; International Space Station (ISS); Random Positioning Machine (RPM); Nucleolus; Electron microscopy; Nucleolin; Microgravity; Magnetic levitation
• ISSN: 0938-0108; 1875-0494
• DOI: 10.1007/s12217-008-9099-z

Changes have been reported in the pattern of gene expression in
Arabidopsis
on exposure to microgravity. Plant cell growth and proliferation are functions that are potentially affected by such changes in gene expression. In the present investigation, the cell proliferation rate, the regulation of cell cycle progression and the rate of ribosome biogenesis (this latter taken to estimate cell growth) have been studied using morphometric markers or parameters evaluated by light and electron microscopy in real microgravity on the International Space Station (ISS) and in ground-based simulated microgravity, using the Random Positioning Machine and the Magnetic Levitation Instrument. Results showed enhanced cell proliferation but depleted cell growth in both real and simulated microgravity, indicating that the two processes are uncoupled, unlike the situation under normal gravity on Earth in which they are strictly co-ordinated events. It is concluded that microgravity is an important stress condition for plant cells compared to normal ground gravity conditions.