Surface behaviour and peptide–lipid interactions of the antibiotic peptides, Maculatin and Citropin

• Published in: Biochimica et biophysica acta Vol. 1664; no. 1; pp. 31 - 37
• Main Authors: Ambroggio, Ernesto E, Separovic, Frances, Bowie, John, Fidelio, Gerardo D
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-07-2004
• Subjects: Adsorption; Amphibian Proteins - chemistry; Anti-Infective Agents - pharmacology; Antibiotic peptide; Antimicrobial Cationic Peptides - chemistry; Hydrogen-Ion Concentration; Kinetics; Lipids - chemistry; Lipid–peptide interaction; Magnetic Resonance Spectroscopy; Monolayer stability; Peptide adsorption; Peptide monolayer; Peptides - chemistry; Phosphatidylcholines - chemistry; Phosphatidylglycerols - chemistry; Thermodynamics; Peptide monolayer; Antibiotic peptide; Lipid–peptide interaction; Peptide adsorption; Monolayer stability
• ISSN: 0005-2736; 0006-3002; 1879-2642
• DOI: 10.1016/j.bbamem.2004.03.013

Surface behaviour of Maculatin 1.1 and Citropin 1.1 antibiotic peptides have been studied using the Langmuir monolayer technique in order to understand the peptide–membrane interaction proposed as critical for cellular lysis. Both peptides have a spontaneous adsorption at the air–water interface, reaching surface potentials similar to those obtained by direct spreading. Collapse pressures (
Π
c, stability to lateral compression), molecular areas at maximal packing and surface potentials (Δ
V) obtained from compression isotherms of both pure peptide monolayers are characteristic of peptides adopting mainly α-helical structure at the interface. The stability of Maculatin monolayers depended on the subphase and increased when pH was raised. In an alkaline environment, Maculatin exhibits a molecular reorganization showing a reproducible discontinuity in the
Π–
A compression isotherm. Both peptides in lipid films with the zwitterionic palmitoyl-oleoyl-phosphatidylcholine (POPC) showed an immiscible behaviour at all lipid–peptide proportions studied. By contrast, in films with the anionic palmitoyl-oleoyl-phosphatidylglycerol (POPG), the peptides showed miscible behaviour when the peptides represented less than 50% of total surface area. Additional penetration experiments also demonstrated that both peptides better interact with POPG compared with POPC monolayers. This lipid preference is discussed as a possible explanation of their antibiotic properties.