Regulated expression of an alcohol dehydrogenase 1 chimeric gene introduced into maize protoplasts

• Published in: Planta Vol. 170; no. 4; pp. 535 - 540
• Main Authors: Howard, E.A. (CSIRO, Canberra (Australia). Div. of Plant Industry), Walker, J.C, Dennis, E.S, Peacock, W.J
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01-04-1987; Springer
• Subjects: Alcohols; Applied sciences; CHIMAERAS; CHIMERE; Corn; Dehydrogenases; DNA; Enzymaktivitaet; Exact sciences and technology; Gene expression regulation; Genes; GENETIC TRANSFORMATION; Gentransformation; Other techniques and industries; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plasmid; Promoter regions; PROTOPLASM; PROTOPLASMA; PROTOPLASME; Protoplast; Protoplasts; QUIMERA; RNA; Transfection; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Zea; ZEA MAYS
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00402988

Transient-expression analysis has shown anaerobic regulation of the alcohol dehydrogenase (Adh1) promoter in a chimeric construct. Chimeric plasmids containing the promoter for the Adh1 gene of maize (Zea mays L.) linked to the coding sequence of the chloramphenicol acetyl transferase (cat) gene were introduced into maize protoplasts using electroporation. Both the introduced Adh1 promoter and the endogenous Adh1 gene promoter are regulated at the RNA level, the O2-tension optimum for induction is the same for both, and both promoters initiate transcription from the same site. The demonstration of regulation of a plant gene promoter in a transient expression system will allow the identification of cis acting sequences responsible for regulation.