Site-specific deletions in the recombinant plasmid pSC101 containing the redB-ori region of phage lambda

Large deletions occur in the hybrid plasmid formed by pSC101 and the EcoRI fragment f2 of phage lambda (redB-ori region) under well defined growth conditions (Bernardi and Bernardi, 1980). We have sequenced the novel joints of the four deletions so obtained and shown that they have one endpoint in p...

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Bibliographic Details
Published in:	Gene Vol. 13; no. 1; p. 103
Main Authors:	Bernardi, A, Bernardi, F
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1981
Subjects:	Bacteriophage lambda - genetics Base Sequence Chromosome Deletion DNA Transposable Elements DNA, Recombinant Hydrogen Bonding Nucleic Acid Conformation Plasmids
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Summary:	Large deletions occur in the hybrid plasmid formed by pSC101 and the EcoRI fragment f2 of phage lambda (redB-ori region) under well defined growth conditions (Bernardi and Bernardi, 1980). We have sequenced the novel joints of the four deletions so obtained and shown that they have one endpoint in pSC101, identical in all four cases, the other endpoint being located in four different lambda sequences. Furthermore, the nucleotide sequences of the novel joints show homologies between the conserved pSC101 sequence and the lambda sequences both conserved and deleted. The presence of an IS-type element in pSC101 is postulated; however, this element is unrelated to the 200 bp element already described in pSC101 (Ravetch et al., 1976).
ISSN:	0378-1119
DOI:	10.1016/0378-1119(81)90047-0