β-Amyloid(25–35) induces the production of interleukin-8 from human monocytes

• Published in: Journal of neuroimmunology Vol. 59; no. 1; pp. 29 - 33
• Main Authors: Meda, Lucia, Bonaiuto, Corrada, Szendrei, Gyorgyi I., Ceska, Miroslav, Rossi, Filippo, Cassatella, marco A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-1995
• Subjects: Alzheimer's disease; Amino Acid Sequence; Amyloid beta-Peptides - pharmacology; Beta-amyloid protein; Cells, Cultured; Humans; Interleukin-8; Interleukin-8 - biosynthesis; Interleukin-8 - genetics; Molecular Sequence Data; Monocytes - metabolism; Mononuclear phagocytes; Peptide Fragments - pharmacology; RNA, Messenger - analysis; Mononuclear phagocytes; Alzheimer's disease; Interleukin-8; Beta-amyloid protein
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/0165-5728(95)00021-S

In an effort to unravel some of the cellular actions of gb-amyloid protein (Aβ), we investigated its effects on interleukin-8 (IL-8) production from human monocytes. Supernatants harvested from cultured monocytes stimulated with the neurotoxic fragment 25-35 of β-amyloid [Aβ(25-35)]contained significant amounts of IL-8. Northern blot analysis demonstrated that Aβ(25-35) also induced IL-8 mRNA accumulation. The effect of Aβ(25–35) on IL-8 mRNA accumulation and secretion was not mimicked by a scrambled Aβ(25–35) peptide, and was not affected by polymyxin B sulphate, which, on the other hand, almost completely abolished the effect of lipopolysaccharide. Our results uncover a new biological action of β-amyloid: that of stimulating the production of a chemokine from monocytes.