Structural analysis of fibrinogen synthesized by cultured chicken hepatocytes in the presence or absence of dexamethasone

• Published in: Biochimica et biophysica acta Vol. 743; no. 3; p. 394
• Main Authors: Amrani, D L, Plant, P W, Pindyck, J, Mosesson, M W, Grieninger, G
• Format: Journal Article
• Language: English
• Published: Netherlands 30-03-1983
• Subjects: Animals; Cells, Cultured; Chick Embryo; Dexamethasone - pharmacology; Enzyme Induction; Fibrinogen - biosynthesis; Fibrinogen - isolation & purification; Liver - drug effects; Liver - enzymology
• ISSN: 0006-3002
• DOI: 10.1016/0167-4838(83)90398-9

Hepatocyte monolayers, derived from chick embryos and cultured in chemically defined medium without hormones, synthesize and secrete fibrinogen that resembles chicken plasma fibrinogen immunochemically and structurally. Addition of a synthetic glucocorticoid, dexamethasone, to the cultured cells resulted in an appreciable and relatively selective increase in fibrinogen synthesis. Autoradiography of fibrinogen that had been metabolically labelled with [35S]methionine and then subjected to SDS-polyacrylamide gel electrophoresis, unreduced or under disulfide-reducing conditions, revealed that only dimeric forms of fibrinogen, containing undegraded A alpha, B beta, and gamma chains, were secreted under stimulated and unstimulated culture conditions.