Paf-acether formation and arachidonic acid freeing from platelet ether-linked glyceryl-phosphorylcholine

• Published in: Biochemical and biophysical research communications Vol. 124; no. 2; p. 637
• Main Authors: Chignard, M, Le Couedic, J P, Coëffier, E, Benveniste, J
• Format: Journal Article
• Language: English
• Published: United States 30-10-1984
• Subjects: Animals; Arachidonic Acid; Arachidonic Acids - blood; Blood Platelets - metabolism; Phospholipids - blood; Platelet Activating Factor - biosynthesis; Platelet Activating Factor - blood; Platelet Aggregation; Rabbits; Tritium
• ISSN: 0006-291X
• DOI: 10.1016/0006-291X(84)91602-4

Ether-linked glyceryl-phosphorylcholines (GPC) are potential precursors for paf-acether biosynthesis. We show that such phospholipids are present in rabbit platelets in sufficient amounts to insure lyso paf-acether and paf-acether formation. Indeed, upon stimulation, platelets formed lyso paf-acether and paf-acether in amounts representing 10% and 0.2% of the total amounts of ether-linked GPC. Following preincubation with [3H]paf-acether, ether-linked GPC labelled at position 1 of the glycerol were detected in platelets. They were hydrolysed, and gave birth to lyso paf-acether upon platelet activation. Platelet ether-linked GPC labelled at position 2 with [3H]arachidonic acid (AA) were also hydrolyzed under the same experimental conditions. These data indicate that in rabbit platelets these phospholipids are precursors for paf-acether and AA, two mediators of platelet activation.