Quantitative analysis of the terminal branches of facial nerve in fresh frozen head and neck specimens

• Published in: Folia morphologica Vol. 73; no. 1; pp. 24 - 29
• Main Authors: Sargon, M F, Ogretmenoglu, O, Gunenc Beser, C, Karaoglan, Y, Ercakmak, B, Hayran, H M, Hayran, M, Kasirga, U B
• Format: Journal Article
• Language: English
• Published: Poland Wydawnictwo Via Medica 01-02-2014
• Subjects: Aged; Aged, 80 and over; Facial nerve; Facial Nerve - anatomy & histology; Facial Nerve - ultrastructure; Female; Fibers; Frozen Sections; Head - innervation; Head and neck; Humans; Male; Mandible; Myelin Sheath - metabolism; Neck - innervation; Quantitative analysis; Statistical analysis
• ISSN: 0015-5659; 1644-3284
• DOI: 10.5603/FM.2014.0004

The first aim of this study was the quantification of nerve fibres found in terminal branches of facial nerve and the second aim was the ultrastructural analysis of these terminal branches in order to observe their ultrastructural differences, if present. In the examination of literature; we could not find any studies related to this subject.
Four fresh frozen head and neck specimens were used and the dissections were done bilaterally. Therefore; totally 8 samples were examined. The samples were prepared according to routine transmission electron microscopic tissue preparation technique. The semi-thin sections were examined under light microscope by camera lucida. In every sample, the quantitative analysis was performed in 5 different areas in an area of 0.01 mm2 and statistical analysis was done. Secondly; the ultrastructural appearance of these terminal branches were examined under transmission electron microscope.
In the quantitative analysis of terminal branches of facial nerve in an area of 0.01 mm2; the least number of nerve fibres were found in temporal branches and the highest number were detected in cervical branches. In transmission electron microscopic examination, no significant difference was found in between these branches. In the statistical analysis; statistically significant differences were obtained in between the temporal and buccal, marginal mandibular, cervical branches; zygomatic and marginal mandibular, cervical branches; buccal and marginal mandibular, cervical branches; marginal mandibular and cervical branches.
These numerical data will have an importance during the nerve repair process of terminal branches of facial nerve in various injuries.