Morphological, structural and cytotoxic behavior of starch/silver nanocomposites with synthesized silver nanoparticles using Stevia rebaudiana extracts

• Published in: Polymer bulletin (Berlin, Germany) Vol. 78; no. 3; pp. 1683 - 1701
• Main Authors: Valera-Zaragoza, M., Huerta-Heredia, A. A., Peña-Rico, M. A., Juarez-Arellano, E. A., Navarro-Mtz, A. K., Ramírez-Vargas, E., Sánchez-Valdes, S.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2021; Springer Nature B.V
• Subjects: Biocompatibility; Biopolymers; Biosynthesis; Breast cancer; Cancer therapies; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Cytotoxicity; Diffraction patterns; Dispersion; Electron microscopes; Glycerol; High resolution electron microscopy; Medical prognosis; Metabolites; Morphology; Nanocomposites; Nanoparticles; Nitrates; Nitrogen; Optical microscopy; Organic Chemistry; Original Paper; Physical Chemistry; Polymer Sciences; Scanning electron microscopy; Silver; Silver chloride; Soft and Granular Matter; Thermogravimetric analysis; Nanocomposites; Starch; Morphology; Silver nanoparticles
• ISSN: 0170-0839; 1436-2449
• DOI: 10.1007/s00289-020-03184-6

The synthesis of silver nanoparticles (AgNPs) using a
Stevia rebaudiana
aqueous extract and their dispersion in starch in order to obtain starch/AgNps nanocomposites are shown in this study. The AgNPs and the starch/AgNPs nanocomposites have been characterized by ultraviolet–visible spectroscopy, optical microscopy, high-resolution transmission electron microscopy, scanning electron microscopy, X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis. Furthermore, the interaction of starch/AgNPs nanocomposites with a MCF-7 breast cancer cell line is analyzed. AgNPs with an average size around 15–20 nm have been obtained. starch/AgNPs nanocomposite morphology changes with the content of silver (3, 5 and 8 mM), from totally separated and dispersed nanoparticles to ribbon-type morphology. The diffraction pattern of the starch/AgNPs-3 nanocomposite indicates the formation of silver chloride nanoparticles (AgClNPs). A cytotoxic behavior of starch/AgNPs-3 nanocomposite on MCF-7 cancer cells with less damage to normal cells is observed. Hence, the high dispersion of the AgNPs or AgClNPs in starch could be influencing the selective cytotoxic behavior. Thus, this study proposes the generation of biocompatible composite materials, with AgNPs immobilized on starch. These materials have selective action against breast cancer cells, where the selectivity is controlled through the AgNPs dispersion on the biopolymer.