Induction of Several Adaptive Enzymes by Actinomycin D

• Published in: Science (American Association for the Advancement of Science) Vol. 146; no. 3644; pp. 661 - 663
• Main Authors: Rosen, Fred, Raina, Prem Nath, Milholland, Richard J., Nichol, Charles A.
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 30-10-1964
• Subjects: Actinomycin; Adrenalectomy; Alanine Transaminase; Antibiotics; Antibody formation; Dactinomycin; Dosage; Enzymes; Gene induction; Hydro-Lyases; Hydrocortisone; Liver; Liver - enzymology; Liver cells; Metabolism; Old Medline; Pharmacology; Protein synthesis; Rats; RNA; Serine; Transaminases; LIVER ENZYMOLOGY; METABOLISM; PHARMACOLOGY; EXPERIMENTAL LAB STUDY; RATS; ALANINE AMINOTRANSFERASE; HYDRO-LYASES; ACTINOMYCIN; HYDROCORTISONE; ADRENALECTOMY; AMINOTRANSFERASES
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.146.3644.661

Although actinomycin D has been used to prevent protein synthesis in experiments of several hours' duration, its effects on the synthesis of adaptive enzymes which are induced over a period of several days have received less attention. Treatment of young rats with doses of actinomycin D, which permitted survival for a period of 5 days, resulted in marked increases in the activities of four hepatic enzymes known to be induced by cortisol: alanine transaminase, tyrosine transaminase, serine dehydrase, and tryptophan pyrrolase. Actinomycin D also induced responses of two of these enzymes in adrenalectomized rats.