A Comparison of Physical, Chemical, Biological and Combined Treatments for Detoxification of Free Gossypol in Crushed Whole Cottonseed

• Published in: Waste and biomass valorization Vol. 12; no. 7; pp. 3965 - 3975
• Main Authors: Soares Neto, Clemente Batista, Conceição, Aparecido Almeida, Gomes, Taísa Godoy, de Aquino Ribeiro, Jose Antonio, Campanha, Raquel Bombarda, Barroso, Paulo Augusto Vianna, Machado, Antony Enis Virginio, Mendonça, Simone, De Siqueira, Felix Gonçalves, Miller, Robert Neil Gerard
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-07-2021; Springer Nature B.V
• Subjects: Animal feed; Animal nutrition; Autoclaving; Biomolecules; Calcium hydroxide; Chemical treatment; Cotton; Crushing; Detoxification; Edible oils; Engineering; Environment; Environmental Engineering/Biotechnology; Feed industry; Feeds; Fermentation; Fungi; Gossypol; High performance liquid chromatography; Industrial Pollution Prevention; Laccase; Lipids; Liquid chromatography; Manganese; Manganese peroxidase; Mushrooms; Nutrition; Original Paper; Peroxidase; Proteins; Renewable and Green Energy; Slaked lime; Solid state fermentation; Trace levels; Waste Management/Waste Technology; Biodegradation; Cottonseed; Macro-basidiomycete; Biomass; Pretreatment; Gossypol
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-020-01290-0

Oilseed plants such as cotton (
Gossypium
sp.) generate abundant biomass residues which contain significant levels of edible oil, crude proteins and other desirable biomolecules for the animal nutrition industry. The application of cottonseed cake in animal feed, a by-product of the cotton industry, is limited due to the natural presence of toxic free gossypol (FG), wherein efficient and cost-effective methods for FG detoxification are necessary. Herein, pretreatment methods for reducing FG in crushed whole cottonseed (CWCS) were compared, with residual FG quantified using a sensitive Ultra High-Performance Liquid Chromatography method for detection at trace levels in cottonseed materials. Physical treatment by autoclaving resulted in up to 96% detoxification of FG, without reduction in crude protein (CP) content. Chemical treatment with 1% and 2% Ca(OH)
2
eliminated FG to as low as 0.04%, although a reduction in CP content was observed. Similarly, native fermentation, whilst reducing FG content by 99.66% after 6 days incubation, also reduced CP content. In combined physical and biological solid-state fermentation (SSF), basidiomycete fungi
Ganoderma lucidum
CC351,
Panus lecomtei
CC40,
Pleurotus ostreatus
CC389,
Pleurotus sapidus
CC28 and
Pycnoporus sanguineus
CC400 all degraded FG in autoclaved CWCS to trace levels often lower than obtained by individual treatments. A reduction in total lipids and increase in CP were also observed, improving nutritional quality. The most efficient fungi,
P. ostreatus
CC389 and
P. lecomtei
CC40
,
secreted considerable laccase and manganese peroxidase enzymes during SSF, potentially involved in FG detoxification. Cost effective, non-polluting, value-adding approaches for FG detoxification offer potential in animal feed industries.
Graphic Abstract