Downregulation of cytokine release by heat preconditioning of livers used for transplantation in rats

• Published in: Digestive diseases and sciences Vol. 50; no. 10; pp. 1823 - 1828
• Main Authors: WATANABE, Jota, KUSHIHATA, Fumiki, MATSUMOTO, Kinya, HONDA, Kazuo, MATSUDA, Seiji, KOBAYASHI, Nobuaki
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-10-2005; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Graft Survival; Hyperthermia, Induced; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-10 - blood; Interleukin-6 - blood; Liver - metabolism; Liver - pathology; Liver Transplantation; Liver, biliary tract, pancreas, portal circulation, spleen; Male; Medical sciences; Rats; Rats, Inbred Lew; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Tissue and Organ Harvesting - methods; Tumor Necrosis Factor-alpha - metabolism; Rat; Liver; Rodentia; Cytokine; ICAM-1; heat preconditioning; Vertebrata; Mammalia; Animal; transplantation; Preconditioning; Release; Liver transplantation
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-005-2945-6

We evaluated the changes in the levels of released cytokines following heat preconditioning of the livers used in rat liver transplantation. The donor rats in the heat preconditioning (HP) group were subjected to heat preconditioning 48 h before graft harvesting. The liver isografts were preserved in Euro-Collins solution for 8 h, and then transplanted orthotopically. The one-week survival rate of the HP group was significantly better than that of the control (C) group. The serum levels of interleukin-6 and interleukin-10 were significantly lower in the HP group than in the C group. Histological staining revealed that the stagnation of red blood cells and infiltration of neutrocytes were reduced in the HP group. The expression of intercellular adhesion molecule-1 was decreased around the central vein in the HP group, as revealed by immunohistochemistry. These results indicate that heat preconditioning downregulates cytokine release and reduces the frequency of microcirculation disorders.