Identification and characterization of aldehyde dehydrogenase (ALDH) gene superfamily in garlic and expression profiling in response to drought, salinity, and ABA

• Published in: Gene Vol. 860; p. 147215
• Main Authors: Munim Twaij, Baan, Jameel Ibraheem, Liqaa, Al-Shammari, Rana Hadi H., Hasan, Mahmudul, Akter Khoko, Roksana, Sunzid Ahomed, Md, Prodhan, Shamsul H., Nazmul Hasan, Md
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-04-2023
• Subjects: Abiotic stress; Aldehyde Dehydrogenase - genetics; Aldehyde Dehydrogenase - metabolism; ALDH; Arabidopsis - genetics; Cis-element; Droughts; Garlic; Garlic - genetics; Gene expression; Gene Expression Regulation, Plant; Multigene Family; Phylogeny; Salinity; Stress, Physiological - genetics; ABA; Abiotic stress; AGNC; qRT-PCR; kDa; Cis-element; Gene expression; FPKM; GEO; ALDH; As; CDS; NCBI; pI; Garlic
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2023.147215

•Thirty-four (34) members of the aldehyde dehydrogenases (ALDHs) have been identified in the garlic genome.•Phylogenetic and motif analyses reveal that the ALDH members exhibit higher diversity and conservation in garlic.•AsALDH genes showed differential expressions, which indicate involvement in the plant's growth and development.
In response to biotic and abiotic stressors, aldehydes are detoxified and converted to carboxylic acids by aldehyde dehydrogenases (ALDHs), which are enzymes that use NAD+/NADP+ as cofactors. Garlic (Allium sativum L.) has not yet undergone a systematic examination of the ALDH superfamily, despite the genome sequence having been made public. In this investigation, we identified, characterized, and profiled the expression of the garlic ALDH gene family over the entire genome. The ALDH Gene Nomenclature Committee (AGNC) classification was used to classify and name the 34 ALDH genes that were discovered. Except for chromosome 8, all AsALDH genes were dispersed across the chromosomes. AsALDH genes have various localizations, according to predictions about subcellular localization. The AsALDH proteins are more varied and closely related to rice than to Arabidopsis, according to a study of conserved motifs and phylogenetic relationships. The presence of stress modulation pathways is indicated by the abundance of stress-related cis-elements in the AsALDH genes' promoter regions. Analysis of the RNA-seq data showed that AsALDHs expressed differently in various tissues and at various developmental stages. Nine AsALDHs were chosen for study using RT-qPCR, and the results revealed that the majority of the genes were upregulated in response to ABA and downregulated in response to salinity and drought. The results of this study improved our knowledge of the traits, evolutionary background, and biological functions of AsALDHs genes in growth and development.