Peroxiredoxins are required for spindle assembly, chromosome organization, and polarization in mouse oocytes

• Published in: Biochemical and biophysical research communications Vol. 489; no. 2; pp. 193 - 199
• Main Authors: Jeon, Hyuk-Joon, Park, Yong Seok, Cho, Dong-Hyung, Kim, Jae-Sung, Kim, Eunji, Chae, Ho Zoon, Chun, Sang-Young, Oh, Jeong Su
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22-07-2017
• Subjects: Animals; Cell Polarity - drug effects; Cells, Cultured; Chromosomes, Mammalian - drug effects; Chromosomes, Mammalian - metabolism; Dose-Response Relationship, Drug; Female; Meiosis; Meiosis - drug effects; Mice; Oocyte; Oocytes - drug effects; Oocytes - metabolism; Peroxiredoxin; Peroxiredoxins - genetics; Peroxiredoxins - pharmacology; Quinoxalines - pharmacology; Spindle Apparatus - drug effects; Spindle Apparatus - metabolism; Structure-Activity Relationship; Peroxiredoxin; Conoidin A (PubChem CID: 511509); Meiosis; Oocyte
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2017.05.127

Peroxiredoxins (Prxs) are highly conserved antioxidant enzymes and are implicated in multiple biological processes; however, their function in oocyte meiosis has not been studied. Here we show that inhibition of Prx I and II results in spindle defects, chromosome disorganization, and impaired polarization in mouse oocytes. Prx I was specifically localized at the spindle, whereas Prx II was enriched at the oocyte cortex and chromosomes. Inhibition of Prx activity with conoidin A disturbed assembly of the microtubule organizing center (MTOC) through Aurora A regulation, leading to defects in spindle formation. Moreover, conoidin A impaired actin filament and cortical granule (CG) distribution, disrupting actin cap and CG formation, respectively. Conoidin A also increased DNA damage without significantly increasing reactive oxygen species (ROS) levels, suggesting that the effects of conoidin A on meiotic maturation are not likely associated with ROS scavenging pathways. Therefore, our data suggest that Prxs are required for spindle assembly, chromosome organization, and polarization during meiotic maturation.
•Conoidin A disturbs spindle assembly and chromosome organization.•Conoidin A disrupts MTOC organization via Aurora A dysregulation.•Conoidin A impairs polarization of oocytes without changing cellular ROS level.