Effect of heat stress on the expression profile of Hsp90 among Sahiwal (Bos indicus) and Frieswal (Bos indicus×Bos taurus) breed of cattle: A comparative study

• Published in: Gene Vol. 536; no. 2; pp. 435 - 440
• Main Authors: Deb, Rajib, Sajjanar, Basavaraj, Singh, Umesh, Kumar, Sushil, Singh, Rani, Sengar, G., Sharma, Arjava
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25-02-2014
• Subjects: Animals; Breeding; Cattle; Cell Survival - genetics; Expression; Frieswal; Heat shock protein 90; Heat stress; Heat-Shock Response - genetics; Hot Temperature; HSP90 Heat-Shock Proteins - genetics; Leukocytes, Mononuclear; RNA, Messenger - genetics; Sahiwal; Transcriptome - genetics; Hsp; ANOVA; Expression; Sahiwal; qPCR; Heat stress; PBMC; Frieswal; HF; ELISA; Heat shock protein 90; peripheral blood mononuclear cells; Holstein Friesian; quantative polymerase chain reaction; heat shock protein; enzyme linked immune sorbent assay; analysis of variants
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2013.11.086

We evaluated the effect of thermal challenge on the expression profile of heat shock protein 90 (Hsp90) among Sahiwal (Bos indicus) and Frieswal (Bos indicus×Bos taurus) breeds of cattle. The present investigation was focused on the comparative studies on Hsp90 expression among Frieswal and Sahiwal under in vitro and environmental heat stress. Measured immediately after the in vitro heat shock to the peripheral blood mononuclear cells (PBMCs), the relative expression of Hsp90 mRNA was significantly (P<0.05) higher in Sahiwal compared to those in Frieswal. In later intervals of time, the differences in the expression levels between the two breeds become negligible coming down towards the basal level. A similar pattern was observed in the protein concentration showing significantly (P<0.05) higher levels in Sahiwal compared to those in Frieswal. The second sets of experiments were undertaken during summer months (March to May) when temperature peaked from 37 to 45°C. During these months, Frieswal cows consistently recorded higher rectal temperatures than the Sahiwal breed. Further during this peak summer stress, Sahiwal showed significantly higher levels of mRNA transcripts as well as protein concentration compared to the Frieswal breed. Our findings also interestingly showed that, the cell viability of PBMC are significantly higher among the Sahiwal than Frieswal. Taken together, the experiments of both induced in vitro and environmental stress conditions indicate that, Sahiwal may express higher levels of Hsp90 then Frieswal to regulate their body temperature and increase cell survivality under heat stressed conditions.
•Sahiwal exhibit a higher level of HSP90 mRNA transcript than Frieswal cows.•The synthesis of HSP90 is comparatively higher among Sahiwal than Frieswal cows.•Under stress the viability of PBMC cells was higher among Sahiwal than Frieswal cows.