Corn-derived alpha-D-glucan nanoparticles as adjuvant for intramuscular and intranasal immunization in pigs

Adjuvant potential of positively charged corn-derived nanoparticles (Nano-11) was earlier revealed in mice. We evaluated its adjuvant role to electrostatically adsorbed inactivated/killed swine influenza virus antigen (KAg) (Nano-11 + KAg) in pigs. Nano-11 facilitated the uptake of KAg by antigen pr...

Full description

Saved in:
Bibliographic Details
Published in:Nanomedicine Vol. 16; pp. 226 - 235
Main Authors: Dhakal, Santosh, Lu, Fangjia, Ghimire, Shristi, Renu, Sankar, Lakshmanappa, Yashavanth Shaan, Hogshead, Bradley T., Ragland, Darryl, HogenEsch, Harm, Renukaradhya, Gourapura J.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-02-2019
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adjuvant potential of positively charged corn-derived nanoparticles (Nano-11) was earlier revealed in mice. We evaluated its adjuvant role to electrostatically adsorbed inactivated/killed swine influenza virus antigen (KAg) (Nano-11 + KAg) in pigs. Nano-11 facilitated the uptake of KAg by antigen presenting cells and induced secretion of proinflammatory cytokines. In pigs vaccinated by an intranasal mist containing Nano-11 + KAg, expression of T-helper 1 and T-helper 2 transcription factors and secretion of cross-reactive influenza antigen-specific mucosal IgA in the nasal cavity were observed. The enhanced frequencies of IFN-γ positive T-helper and cytotoxic T-cells in Nano-11 + KAg-vaccinates after heterologous virus challenge were also observed. Clinically, slightly reduced influenza signs and pneumonic lesions, with mild reduction in virus load in the respiratory tract of vaccinates were observed. In pigs immunized with Nano-11 adsorbed ovalbumin administered by intramuscular (IM) route, enhanced IgG1 and IgG2 antibodies were detected in serum. Thus, Nano-11 vaccine delivery system confers adjuvant effect in pigs. Sweet corn-derived positively charged nanoparticles (Nano-11) showed adjuvant property in pigs. Nano-11 enhanced antigen uptake by porcine antigen presenting cells and induced the secretion of innate and proinflammatory cytokines in vitro. In vivo Nano-11 significantly improved antigen specific IgG and IgA antibody responses in intramuscular and intranasal immunized pigs, respectively. Hence, Nano-11 can be considered as an alternative adjuvant to use in vaccine formulations. [Display omitted]
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2018.12.003