The combined effect of IL-17F and CCL20 gene polymorphism in susceptibility to multiple sclerosis in Egypt

• Published in: Gene Vol. 685; pp. 164 - 169
• Main Authors: El Sharkawi, Fathia Z., Ali, Sahar A., Hegazy, Mohamed I., Atya, Hanaa B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-02-2019
• Subjects: CCl20; Egyptian; IL-17F; Multiple sclerosis; Polymorphism; RRMS; Multiple sclerosis; IL-17F; BBB; OR; CCL20; SPMS; MS; CNS; Egyptian; SNPs; CCR6; EDSS; Polymorphism; CCl20
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2018.11.006

Levels of CCL20 and its CCR6 receptor are elevated in many autoimmune diseases which help in the recruitment of T helper (Th17) cells to site of inflammation.
Determine the value of single nucleotide polymorphism of CCL20 (rs6749704) and IL-17F (rs763780) genes and their concomitant effect on the serum CCL20 level and susceptibility to MS in Egyptian patients.
Blood samples were collected from 83 patients and 95 healthy subjects. Serum levels of CCL20 were measured by ELISA. The DNA was analyzed for rs6749704 and rs763780 using Genotyping Taqman assay.
The mean serum levels of CCL20 in the MS group were significantly higher than healthy group (P < 0.001). Frequencies of CT genotype of rs6749704 in CCL20 gene and C allele in MS patients were significantly higher compared to controls. Also significant increase of rs763780 in IL-17F gene was detected in MS patients. Concomitant polymorphism in both genes in MS patients showed an increase risk to MS rather than individual locus.
CCL20 may play an important role in the pathogenesis of MS. Both allelic variation of (rs6749704) within CCL20 gene and (rs763780) within IL-17F gene can be considered risk factor for development of MS in Egyptian patients.