Upregulation of circ-0000745 in acute lymphoblastic leukemia enhanced cell proliferation by activating ERK pathway

• Published in: Gene Vol. 751; p. 144726
• Main Authors: Liu, Xiaoliu, Zhou, Chuchan, Li, Yongjian, Deng, Yurong, Lu, Wensheng, Li, Juan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-08-2020
• Subjects: Acute lymphoblastic leukemia; Cell proliferation; Circ-0000745; circRNAs; ERK pathway; Cell proliferation; Circ-0000745; ERK pathway; ALL; circRNAs; Acute lymphoblastic leukemia
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2020.144726

•Circ-0000745 is upregulated in acute lymphoblastic leukemia cells.•Upregulated the expression of circ-0000745 enhanced cell proliferation.•Circ-0000745 activated ERK pathways.
Acute lymphoblastic leukemia (ALL) is a common hematologic malignancy. Circular RNAs (circRNAs) have been reported as an important factor in regulating cellular process expressed in all hematopoietic compartment. But the molecular mechanism of circRNAs in ALL remain unclear. In this study, we observed circ-0000745 upregulated in leukemia cells (Kasumi-1 and KG-1). Upregulated the expression of circ-0000745 significantly enhanced the proliferation of Kasumi-1 and KG-1 cells, and reduced the ratio of apoptosis cells. Knock down the endogenous expression of circ-0000745 suppressed the cell proliferation and increased the ratio of apoptosis cells. Furthermore, western blot assay showed that overexpression of circ-0000745 increased the activity of ERK1/2. Altogether, these results revealed that upregulated circ-0000745 in leukemia cells could promoter cell viability by increasing the activation of ERK pathway. This study supported the important of circRNAs in the process of acute lymphoblastic leukemia, and provided a new biomarker on ALL diagnosis and therapy.