Digestive enzymes of the Californian two-spot octopus, Octopus bimaculoides (Pickford and McConnaughey, 1949)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 215; pp. 10 - 18
• Main Authors: Ibarra-García, Laura Elizabeth, Tovar-Ramírez, Dariel, Rosas, Carlos, Campa-Córdova, Ángel Isidro, Mazón-Suástegui, José Manuel
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-01-2018
• Subjects: Amylases; Amylases - metabolism; Animals; Chymotrypsin - metabolism; Divalent ions; Gastric Juice - enzymology; Inhibitors; Lipase - metabolism; Lipases; Octopodiformes - metabolism; Octopus bimaculoides; Proteases; Salivary Glands - enzymology; Temperature; Trypsin - metabolism; PEPST A; Temperature; DG; GJ; TPCK; Octopus bimaculoides; TLCK; EDTA; Phe; Lipases; Divalent ions; Amylases; Inhibitors; SG; PMSF; Proteases; pH; SIT
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2017.10.001

Octopus bimaculoides is an important commercially fished species in the California Peninsula with aquaculture potential; however, to date limited information is available regarding its digestive physiology. The objective of this study was focused on biochemically characterizing the main enzymes involved in the digestion of O. bimaculoides. Optimum pH, temperature and thermostability were determined for amylases, lipases, trypsin and chymotrypsin; optimum pH and protease inhibitor effect were assessed for acidic and alkaline proteases, and the effect of divalent ions on trypsin and chymotrypsin activity was evaluated in enzymatic extracts from the digestive (DG) and salivary glands (SG) and crop gastric juices (GJ). High amylase activity was detected in GD and GJ whereas this activity is very low in other cephalopods. Salivary glands had the greatest activity in most of the enzyme groups, showing the importance of this organ in digestion. Optimum pH was different depending on the organ and enzyme analyzed. The optimum pH in DG was 3 showing the predominance of acidic proteases in the digestion process. All enzymes were resistant and stable at high temperatures in contrast with other marine species. Trypsin and chymotrypsin activity were highly incremented with the presence of Mg2+, Co2+, Cu2+ and Zn2+ in some tissues. The inhibitor assay showed the importance of serine proteases, metalloproteases and aspartic proteases in the digestive process of this species. This study is the first in assessing the main digestive enzymes of O. bimaculoides and in remarking the importance of other digestive enzyme groups besides proteases in octopuses.