Association of p53 tumor suppressor gene with paraclinical and clinical modalities of gliomas patients in Malaysia

• Published in: Acta neurochirurgica Vol. 146; no. 6; pp. 595 - 601
• Main Authors: Yusoff, A A, Abdullah, J, Abdullah, M R, Mohd Ariff, A R, Isa, M N
• Format: Journal Article
• Language: English
• Published: Austria Springer Nature B.V 01-06-2004
• Subjects: Adolescent; Adult; Amino Acid Substitution; Asian Continental Ancestry Group - genetics; Astrocytoma - diagnosis; Astrocytoma - genetics; Astrocytoma - surgery; Base Pairing - genetics; Brain Neoplasms - diagnosis; Brain Neoplasms - genetics; Brain Neoplasms - surgery; Cancer; DNA Mutational Analysis; Exons; Female; Frameshift Mutation - genetics; Genes, Tumor Suppressor; Glioblastoma - diagnosis; Glioblastoma - genetics; Glioblastoma - surgery; Humans; Magnetic Resonance Imaging; Malaysia; Male; Multivariate analysis; Mutation; Point Mutation - genetics; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Prognosis; Prospective Studies; Tomography, X-Ray Computed; Tumor Suppressor Protein p53 - genetics; Malaysia
• ISSN: 0001-6268; 0942-0940
• DOI: 10.1007/s00701-004-0250-3

Alteration of the tumor suppressor gene p53 is considered to be a critical step in the development of human cancer. Changes in this gene have been detected in a wide range of human tumours, including gliomas. In glioma, the presence of p53 gene alterations has been associated with worse prognosis.
Forty-seven Malaysian adult glioma patients of the Malay race were prospectively studied over a period of 3 years where the presence of p53 mutation using cold-SSCP method and their clinical and paraclinical response were correlated.
Among these glioma patients, p53 mutations were detected in 12 tumors, an incidence rate of 25.5%. Mutations were found in 2 patients of grade II, and 5 patients both in grade III and grade IV. The sequencing results revealed the presence of base-substitutions (7) (58.3%) and frameshifts mutations (5) (41.7%). Of the base-substitutions, 57.1% were transversions and 42.9% were transitions.
Our analysis shows that 3 factors were associated with p53 mutations i.e. grade, site and consistency of tumour using univariate analysis although multivariate analysis revealed no positive on predictors of mutation. In conclusion, although p53 genetic alterations are involved in glioma patients in Malaysia, it has no impact on prognosis.