Quantitative determination of characteristic components from compound of Lysionotus pauciflorus Maxim. by LC–MS/MS and its application to a pharmacokinetic study

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 177; p. 112835
• Main Authors: Liang, Caijuan, Yin, Jintuo, Ma, Yinling, Zhang, Xia, Zhang, Lantong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05-01-2020
• Subjects: Administration, Oral; Animals; Caffeic Acids - administration & dosage; Caffeic Acids - blood; Caffeic Acids - pharmacokinetics; Chromatography, High Pressure Liquid - methods; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - pharmacokinetics; Flavones - administration & dosage; Flavones - blood; Flavones - pharmacokinetics; Glucosides - administration & dosage; Glucosides - blood; Glucosides - pharmacokinetics; Lamiales - chemistry; LC–MS/MS; Male; Models, Animal; Nevadensin; Pharmacokinetics; Phenylpropionates - administration & dosage; Phenylpropionates - blood; Phenylpropionates - pharmacokinetics; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Rosemary acid; Rosmarinic Acid; Salviaflaside; Spectrometry, Mass, Electrospray Ionization - methods; Tablets; Tandem Mass Spectrometry - methods; Tuberculosis, Lymph Node - drug therapy; Rosemary acid; Nevadensin; Pharmacokinetics; Salviaflaside; LC–MS/MS
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2019.112835

•Tuberculosis of cervical lymph nodes is called scrofula in Traditional Chinese Medicine. Clinical manifestation is that unilateral or bilateral neck can have multiple enlarged lymph nodes of different sizes.•Compound of Lysionotus pauciflorus Maxim. have a significant effect on tuberculosis of cervical lymph nodes.•A selective and sensitive LC–MS/MS method is developed and validated in rat plasma for the first time.•The major advantages of the present method are small plasma volume, excellent sensitivity and a short run time.•The pharmacokinetic parameters and plasma concentration-time profiles would prove valuable in pre-clinical and clinical investigations on the disposition of compound medicine.
Tuberculosis of cervical lymph nodes is called scrofula in Traditional Chinese Medicine (TCM). Clinical manifestation is that unilateral or bilateral neck can have multiple enlarged lymph nodes of different sizes. Current therapeutic drugs include Lysionotus pauciflorus Maxim. tablets and compound of Lysionotus pauciflorus Maxim., which have a significant effect on tuberculosis of cervical lymph nodes. This compound is composed of three herbs, Lysionotus pauciflorus Maxim., Prunella vulgaris L. and Artemisia argyi Levl.et Vant. A selective and sensitive LC–MS/MS method was established and validated in rat plasma for the first time. Chromatographic separation was achieved on a Wonda Cract ODS-2 C18 Column (150 mm × 4.6 mm, 5 μm). The mobile phase contained 0.1% formic acid aqueous solution and acetonitrile with a flow rate of 0.8 mL/min. The detection was performed in negative electrospray ionization mode and the precursor/product ion transitions of six components and internal standard (IS) sulfamethoxazole were quantified in multiple reaction monitoring (MRM) using QTRAP-3200 MS/MS. The method fulfilled US Food and Drug Administration guidelines for selectivity, sensitivity, accuracy, precision, matrix effect, extraction recovery, dilution integrity, and stability. This proposed method was then successfully applied to a pharmacokinetic study after oral administration of 10 mL/kg compound extracts in rats. The pharmacokinetic parameters and plasma concentration-time profiles would prove valuable in pre-clinical and clinical investigations on the disposition of compound medicine.