Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homodupl...

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Bibliographic Details
Published in:	Molecular bioSystems Vol. 8; no. 7; p. 1861
Main Authors:	Heinze, Roger J, Sekerina, Svetlana, Winkler, Ines, Biertümpfel, Christian, Oretskaya, Tatiana S, Kubareva, Elena, Friedhoff, Peter
Format:	Journal Article
Language:	English
Published:	England 06-07-2012
Subjects:	Base Pair Mismatch DNA Mismatch Repair DNA, Bacterial - chemistry DNA, Bacterial - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism MutS DNA Mismatch-Binding Protein - chemistry MutS DNA Mismatch-Binding Protein - metabolism Nucleic Acid Heteroduplexes - genetics Nucleic Acid Heteroduplexes - metabolism Signal Transduction
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Summary:	The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.
ISSN:	1742-2051
DOI:	10.1039/c2mb25086a