Description and analysis of genetic diversity between commercial bean lines (Phaseolus vulgaris L.)

• Published in: Theoretical and applied genetics Vol. 101; no. 8; pp. 1207 - 1214
• Main Authors: METAIS, I, AUBRY, C, HAMON, B, JALOUZOT, R, PELTIER, D
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-12-2000; Berlin Springer Nature B.V; Springer Verlag
• Subjects: Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetic diversity; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Germplasm; Life Sciences; Phaseolus vulgaris; Polymorphism; Pteridophyta, spermatophyta; Vegetals; United States > US; Grain legume; Leguminosae; Genetic marker; Minisatellite DNA; Phaseolus vulgaris; Dicotyledones; Angiospermae; Spermatophyta; Genetic distance; Genetic diversity; Polymorphism; RAPD; ISSR
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051599

The effectiveness of RFLP, DAMD-PCR, ISSR and RAPD markers in assessing polymorphism and relationships between 24 commercial lines of Phaseolus vulgaris L.was evaluated. We have used a Phaseolus-specific minisatellite sequence as a probe, which enabled 23 of the bean lines tested to be fingerprinted. Based on the sequence information obtained, primers corresponding to the bean-specific minisatellite core sequence were used in subsequent PCR amplifications. Our observations indicated that while the DAMD-PCR was sensitive in detecting genetic variation between bean species and between accessions of P. vulgaris, when used alone it may be limited in its ability to detect genetic variation among cultivated bean lines due to the low number of loci amplified. Only one out of the five ISSR primers tested was efficient in generating multiple band profiles, which was insufficient to distinguish all the different bean lines. Reproducible RAPD profiles were obtained, and these allowed us to differentiate all the genotypes tested with seven primers. We ultimately used only results from RFLP and RAPD markers to explore the genetic diversity among commercial bean lines. Both analyses led to the same clustering of the bean lines according to their geographical origins (United States or Europe). With respect to the European lines, the results obtained from RAPD data also enable the lines to be clustered according to their creators.[PUBLICATION ABSTRACT]